Physical, Thermal and Morphology Properties af Polyvinyl Alcohol-Based Composite Enhanced with Collagen and Bromelain for Cartilage Application

Abstract

This thesis reports the preparation of polyvinyl alcohol (PVA) film modified with
collagen and 3% bromelain via solvent casting and surface entrapment method for
cartilage application. The concentration of collagen used was varied from 0 to 2% w/v
the hydrophilicity properties of the film was observed via swelling test and moisture
absorption test. PVA/2%Col with 3% bromelain having good swelling properties in
phosphate buffer saline (PBS) solution and have a good results of moisture absorption
compared to other films. PVA/2%Col with 3% bromelain concentration was chosen as
the optimum PVA/Collagen film and was further tested in order to observe its physical
properties. The chemical interaction of the film was analyzed by Fourier Transfrom
Infrared Spectroscopy (FTIR). Scanning Electron Microscope (SEM) testing also have
been conducted to observe it surface morphology. Differential Scanning Calorimetry
(DSC) testing, PVA/2%Col with 3% bromelain film give a better results compared to
other films. Futhermore, for In-vitro Biomineralization testing using SEM,
PVA/2%Col with 3% bromelain shows good results. These report were clearly
suggestive that biopolymers such as PVA can exhibit an increasing of physical
properties by treated with collagen and bromelain. This new modified towards the
PVA film can enhance its quality and decrease the limitations of application in treating
cartilage application.